Amyloglucosidase Suppresses Interference by Glycogen in the Quantification of DNA Using the Hoechst 33258 Dye
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چکیده
منابع مشابه
Amyloglucosidase suppresses interference by glycogen in the quantification of DNA using the Hoechst 33258 dye.
The Hoechst dye fluorescence technique, which is used for the quantitative determination of DNA (12), requires high saline concentrations at neutral pH to reach maximal fluorescence of the DNA-dye complex and to render the DNA fully accessible to the dye by dissociating foreign molecules (3,12). In conventional assays, the DNA is alcohol-precipitated and then resuspended in buffer containing 2 ...
متن کاملQuantitation of DNA using Hoechst 33258
An essential element of cellular and molecular biology is the ability to quantitate DNA in large numbers of samples at a sensitivity that enables determination of small amounts of sample. Here we describe a fluorescent method to quantitate DNA with Hoechst 33258 dye and the BioTek FL600 fluorescence microplate reader. This assay provides linear results over three orders of magnitude and can rou...
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OBJECTIVE To compare two fluorometric assays, utilizing (1) the bisbenzimidazole Hoechst 33258 and (2) PicoGreen, for determining DNA content in human cartilage. METHODS Human articular and nasal septal cartilage explants were digested using proteinase K. Portions of sample digest were analysed for intrinsic and dye-enhanced fluorescence with either Hoechst 33258 or PicoGreen. RESULTS Intri...
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Measurement of cell numbers in microtiter culture plates using the fluorescent dye Hoechst 33258.
A sensitive fluorimetric assay based on DNA binding of the fluorescent dye Hoechst 33258 was developed to quantitate cell numbers in microtiter plates. The cells were fixed and incubated with the dye solution. The bound dye was extracted with ethanol and fluorescence was measured in a spectrofluorimeter. The method is sensitive for as little as a thousand cells and is particularly useful for th...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2002
ISSN: 0736-6205,1940-9818
DOI: 10.2144/02326bm07